ABSTRACT
<p><b>OBJECTIVE</b>The correlation were studied between testosterone 5-alpha-reductase II (SRD5A2) gene polymorphisms and prognosis factors.</p><p><b>METHODS</b>V89L and A49T variants was identified with Mwo1 and Rsa1. The differences of V89L and A49T between cancer of prostate (CaP) and benign prostatic hyperplasia (BPH) were studied. In addition, we also researched the association of polymorphisms with age of onset, free prostate specific antigen (FPSA), total PSA (TPSA), FPSA/TPSA (F/T), Gleason score, and T stage in cancer group.</p><p><b>RESULTS</b>We found no differences of V89L and A49T polymorphisms between CaP and BPH. In CaP group the A49T variant was associated with lower age of onset (P = 0.03) and higher Gleason score (P = 0.015). There were no differences between VV and VL+LL polymorphisms with any of the characteristics studied. When the characteristics above were regarded as two-level discrete variable, there were no differences by A49T and V89Lvariants.</p><p><b>CONCLUSION</b>In CaP group, the AT+TT genotype was perhaps associated with poor prognosis. VL+LL genotype has no relation with prognosis.</p>
Subject(s)
Aged , Aged, 80 and over , Humans , Male , Middle Aged , 3-Oxo-5-alpha-Steroid 4-Dehydrogenase , Genetics , Gene Frequency , Genetic Predisposition to Disease , Genotype , Neoplasm Staging , Polymorphism, Genetic , Prognosis , Prostate-Specific Antigen , Blood , Prostatic Hyperplasia , Genetics , Prostatic Neoplasms , Blood , Genetics , PathologyABSTRACT
<p><b>OBJECTIVE</b>To construct and screen the suppression subtractive hybridization (SSH) library of human renal cell carcinoma (RCC).</p><p><b>METHODS</b>Poly A(+) RNA was isolated from RCC lines 786-O (tester) and renal cell (RC) lines HK-2 (driver), respectively. SSH procedure was performed according to the protocol of the PCR-Select cDNA Subtraction Kit (Clontech), and PCR products were cloned into pT-Adv vector and transformed E. coli TOP10F'. All positive clones picked out were digested and some of which were sequenced.</p><p><b>RESULTS</b>The SSH library contained 362 clones with SSH cDNA fragments distributed mainly from 0.3 to 0.9 kb. Among 50 clones sequenced randomly, 2 represented unknown genes and the other 48 derived from 36 known genes.</p><p><b>CONCLUSION</b>The quality of the SSH library of human RCC is reliable and its construction is the basis for further screening differentially expressed genes of RCC.</p>
Subject(s)
Humans , Adenocarcinoma, Clear Cell , Genetics , Cell Line, Tumor , Gene Library , Kidney Neoplasms , Genetics , Nucleic Acid Hybridization , MethodsABSTRACT
<p><b>OBJECTIVE</b>To evaluate the in vitro and in vivo function of anti-human bladder tumor human-mouse chimeric antibody ch-BDI and its future clinical application.</p><p><b>METHODS</b>With ch-BDI in high-expression cell-line medium, affinity chromatography was used for the purification. Labeled with (99m)Tc through reduction method, its immunoreactive fraction and association constant were measured. The constant was injected into nude mice with xenografted human bladder tumor. The biodistribution of the labeled ch-BDI was studied with radioimmunoimaging.</p><p><b>RESULTS</b>ch-BDI showed desirable immunoreactive fraction (76%) and association constant (3.56 x 10(9) M(-1)) in vitro and a terrific specific targeting effect in vivo.</p><p><b>CONCLUSION</b>ch-BDI has fairly good function against human bladder tumor both in vitro and in vivo, and is promising in clinical use.</p>